THE B AND y CYTOPLASMIC ACTINS ARE DIFFERENTIALLY THERMOSTABILIZED BY MgADP; y ACTIN BINDS MgADP MORE STRONGLY
نویسنده
چکیده
N. Leigh Anderson Molecular Anatomy Program Division of Biological and Medical Research Argonne National Laboratory Argonne, Illinois 60439 Received June 6,1979 SUMMARY: Using the technique of thermal denaturation in a temperature gradient followed by two-dimensional gel electrophoresis of the fractions, the thermostabilities of the cytoplasmic actins have been investigated. Addition of 5 mM MgADP (but not NADH or CaC12) to stripped, radiolabeled leukocyte lysate thermostabilizies the actins by about 16'C relative to stripped lysate alone, producing a slightly stronger stabilization in y actin. This result will allow preparative fractionation of B and y actins and additional biochemical characterization of their differences.
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